Tellurite-reduction-based assay for screening potential antibiotics
نویسندگان
چکیده
منابع مشابه
A competition-based assay for the screening of species-specific antibiotics.
OBJECTIVES To develop a high throughput screening-compatible assay for the selection of species-specific antibiotics that do not harm human cells. METHODS Staphylococcus aureus and human reporter cells continuously generating a fluorescence signal were competitively co-cultivated. The fluorescence signals were determined in the presence and absence of the specific antibiotic streptomycin and ...
متن کاملSelenite and tellurite reduction by Shewanella oneidensis.
Shewanella oneidensis MR-1 reduces selenite and tellurite preferentially under anaerobic conditions. The Se(0) and Te(0) deposits are located extracellularly and intracellularly, respectively. This difference in localization and the distinct effect of some inhibitors and electron acceptors on these reduction processes are taken as evidence of two independent pathways.
متن کاملComparison of Microscopy and Alamar Blue Reduction in a Larval Based Assay for Schistosome Drug Screening
BACKGROUND In view of the current widespread use of and reliance on a single schistosomicide, praziquantel, there is a pressing need to discover and develop alternative drugs for schistosomiasis. One approach to this is to develop High Throughput in vitro whole organism screens (HTS) to identify hits amongst large compound libraries. METHODOLOGY/PRINCIPAL FINDINGS We have been carrying out lo...
متن کاملHigh-throughput screening for Kv1.3 channel blockers using an improved FLIPR-based membrane-potential assay.
Voltage-gated K(+) channels are potential drug targets for an increasing number of disease indications. Searching for compounds that modulate K(+) channel activities by high-throughput screening (HTS) is becoming a standard approach in the drug discovery effort. Here the authors report an improved fluorometric imaging plate reader (FLIPR) membrane potential assay for Kv1.3 K(+) channel HTS. The...
متن کاملIdentification of Novel Potential Antibiotics against Staphylococcus Using Structure-Based Drug Screening Targeting Dihydrofolate Reductase
The emergence of multidrug-resistant Staphylococcus aureus (S. aureus) makes the treatment of infectious diseases in hospitals more difficult and increases the mortality of the patients. In this study, we attempted to identify novel potent antibiotic candidate compounds against S. aureus dihydrofolate reductase (saDHFR). We performed three-step in silico structure-based drug screening (SBDS) ba...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2012
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.035113-0